Produkty

Agilent
Bio MAb

Agilent
Bio IEX

Agilent
Bio SEC-3

Agilent
Bio SEC-5

Column Type

Ion Exchange

Ion Exchange

Size Exclusion

Size Exclusion

DataSheet
Bio MAb
(0.04 MB)

DataSheet
Bio IEX
(0.04 MB)

DataSheet
Bio SEC-3
(0.04 MB)

DataSheet
Bio SEC-5
(0.04 MB)

Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms.

For high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins.

Available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

Available up to 2000Å pore size for a broad range of size-based, bio-molecule separations.

Phase

WCX (COOH)

SCX (SO3H)
WCX (COOH)
SAX (N(CH3)3)
WAX (N(C2H5)2)

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Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a weak cation exchange layer

Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating  and bonded with an ion exchange layer

Spherical, high purity, porous silica with a hydrophilic polymeric coating

Spherical, high purity, porous silica with a hydrophilic polymeric coating

1.7, 3, 5, and 10 μm

1.7, 3, 5, and 10 μm

3 μm

5 μm

Non-porous

Non-porous

100Å, 150Å, 300Å

100Å, 150Å, 300Å, 500Å, 1000Å, 2000Å

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100Å MW: 100-100,000 Da
150Å MW: 500-150,000 Da
300Å MW: 5,000-1,250,000 Da

100Å MW: 100-100,000 Da
150Å MW: 500-150,000 Da
300Å MW: 5,000-1,250,000 Da
500Å MW: 15,000-5,000,000 Da
1000Å MW: 50,000-7,500,000 Da
2000Å MW: >10,000,000 Da

2 - 12

2 - 12

2 - 8.5

2 - 8.5

80°C

80°C

Recommended range: 10-30°C, maximum: 80°C

Recommended range: 10-30°C, maximum: 80°C

Pressure Limit (column hardware)

600 bar (8,700 psi) for stainless steel hardware
400 bar (5,800 psi) for PEEK hardware

600 bar (8,700 psi) for stainless steel hardware
400 bar (5,800 psi) for PEEK hardware

Recommended operating pressure: 137 bar (2,000 psi)
Maximum pressure: 240 bar (3,500 psi)

Recommended operating pressure: 137 bar (2,000 psi)
Maximum pressure: 240 bar (3,500 psi)

Compatible with aqueous buffers, acetonitrile, acetone, methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate.

Compatible with aqueous buffers, acetonitrile, acetone, methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate.

Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used

Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used

Typical 0.1-1.0 mL/min for a 4.6 mm ID column

Typical 0.1-1.0 mL/min for a 4.6 mm ID column

0.1 - 1.25 mL/min for 7.8 mm ID columns
0.1 - 0.4 mL/min for 4.6 mm ID columns

0.1 - 1.25 mL/min for 7.8 mm ID columns
0.1 - 0.4 mL/min for 4.6 mm ID columns