| Copy Number Analysis | |
| Product/Application | Description | Download/Discover |
| Automating the CGH/CNV Workflow with the Bravo Automated Liquid Handling Platform | A new method was developed to automate CGH/CNV sample fragmentation, labeling, purification, and pre-hybridization setup in a 96-well format by using the Agilent Bravo Automated Liquid Handling Platform. Protocols for both the standard enzymatic (Genomic DNA Enzymatic Labeling Kit) and ULS (Genomic DNA High-Throughput ULS Labeling Kit) methodologies have been developed and validated with VWorks Automation Control software. The new workflow, compatible with both SurePrint HD and G3 microarrays, enables superior performance across the entire Agilent CGH/ CNV portfolio. Throughput is increased and hands-on time is decreased, with no compromise in data quality. | > Download Application Note > Discover Agilent Bravo liquid
handling platform > Discover Agilent CGH product
portfolio > Discover Agilent CNV product
portfolio |
| Protocol for Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis using Bravo Automated Liquid Handling Platform with Enzymatic and ULS Labeling | This guide describes Agilent's recommended operational procedures to analyze DNA copy number variations using Agilent 60-mer oligonucleotide microarrays for array- based comparative genomic hybridization (aCGH) analysis. This protocol is specifically developed and optimised to use the Bravo Automated Liquid Handling Platform for the labeling (ULS and enzymatic), purification and pre-hybridization steps. | > Download Protocol |
| qPCR/PCR |
| Product/Application | Description | Download/Discover |
| Complete Automation of Quantitative PCR Setup on the Agilent Bravo Automated Liquid Handling Platform | The Agilent Bravo Automated Liquid Handling Platform has been shown to be an effective way to automate setup of a variety of quantitative polymerase chain reaction (QPCR) assays. QPCR reactions to examine a variety of housekeeping genes from cDNAs derived from different human tissues were set up on the Bravo Automated Liquid Handling Platform and by hand. Dilutions performed by each method showed similar slopes and good linearity and targets amplified by either method also gave similar Ct values. When examining cDNAs from fetal and adult lung, differential expression was observed in the B2M whereas GUSB and TBP remained similar. The Bravo Automated Liquid Handling Platform should be useful to scientists interested in speeding up setup while maintaining reliability when performing QPCR studies. | > Download Application Note > Discover Agilent Bravo liquid
handling platform > Discover Stratagene qPCR and
RT-qPCR reagents |
| Complete Automation of the Stratagene StrataPrep 96 PCR Purification Kit with the Agilent Bravo Automated Liquid Handling Platform and Agilent Automated Centrifuge | A new method was developed to automate PCR purification in a 96-well format using the Bravo Automated Liquid Handling Platform, Automated Centrifuge and StrataPrep 96 PCR Purification Kit from Agilent Technologies (Automation Solutions and Stratagene divisions, respectively). Current PCR purification protocols require extensive manual processing including repetitive pipetting and vacuum filtration steps. Using Agilent’s VWorks Automation Control software, a protocol was developed for the Bravo Platform to aspirate, dispense, mix, and transfer sample from a source 96-well PCR plate to the binding and collection plates of the StrataPrep 96 kit as well as to automate sample wash steps using an accessory vacuum filtration unit on the Bravo Platform. Sample recovery was accomplished using the Agilent Automated Centrifuge. DNA recovery quality was assessed on the Agilent BioAnalyzer 2100. | > Download Brochure > Download Application Note > Discover Agilent Bravo liquid
handling platform > Discover Agilent Microplate
Centrifuge > Discover Stratagen StrataPrep®
96 PCR Purification Kit > Discover Agilent 2100 BioAnalyzer |
| Promega Wizard SV96 PCR cleanup on the BenchCel Workstation | Agilent Automation provides a complete benchtop solution for PCR Clean-up. The combination of the Bravo Automated Liquid Handling Platform and the BenchCel Microplate Handler allows for a flexible, efficient and compact approach to the automation of PCR Clean-up. The BenchCel Microplate Handler has capacity to deliver 15 kits to the Bravo Liquid Handler for processing. The Bravo Liquid Handler automatically assembles and disassembles the vacuum manifold through multiple elution cycles without user interaction for true walk-away performance. | > Download Application Highlight > Discover Agilent Bravo liquid
handling platform > Discover Agilent BenchCel
Microplate Handler > Discover Agilent Mx3000P™ Real-
Time PCR System |
| Next-Generation Sequencing |
| Product/Application | Description | Download/Discover |
| Automation of Agencourt AMPure Purification Kit for the Purification of Next-Generation Sequencing Sample Preparation Reactions on Bravo | A new method was developed to automate library construction for the Illumina sequencing platform using the Agilent Bravo Automated Liquid Handling Platform and the Agencourt Ampure PCR purification kit. Current Next-Generation sequencing workflows require extensive manual processing steps including repetitive pipetting and the use of individual centrifugation columns for cleanups of enzymatic steps. Using the Agilent VWorks Automation Control software, a protocol was developed for the Bravo platform to aspirate and dispense enzyme master-mixes, and perform cleanups of these reactions through the transfer of the Agencourt AMPure reagent to the reaction, transfer of the microtiter plate to a magnet using the integrated gripper, and subsequent sample wash and elution steps. Sample quality and quantity were assessed using the Agilent Bioanalyzer 2100 and Illumina Genome Analyzer IIX. | > Download Application Note > Discover Agilent Bravo liquid
handling platform > Discover Agilent 2100 BioAnalyzer > Discover Agilent High Sensitivity
DNA Kit > Discover Agilent Target
Enrichment products |
| RNA / DNA sample prep |
| Product/Application | Description | Download/Discover |
| Automation of Stratagene Absolutely RNA 96 Microprep Kit with the Bravo Automated Liquid Handling Platform | A new method was developed to automate RNA isolation in a 96-well format using the Bravo Automated Liquid Handling Platform and Absolutely RNA 96 Microprep Kit from Agilent Technologies (Automation Solutions and Stratagene divisions, respectively). Current RNA isolation protocols require extensive manual processing including repetitive pipetting and vacuum filtration steps. Using Agilent’s VWorks Automation Control software, a script was written for the Bravo Platform to aspirate, dispense, mix, and transfer sample from a source 96-well tissue culture plate to the binding and collection plates of the Absolutely RNA 96 kit as well as to automate sample wash steps using an accessory vacuum filtration unit on the Bravo Platform. RNA quality was assessed on the Agilent BioAnalyzer 2100. | > Download Application Note > Discover Agilent Bravo liquid
handling platform > Discover Agilent Microplate
Centrifuge > Discover Stratagene Absolutely
RNA® 96 Microprep Kit > Discover Agilent 2100 BioAnalyzer > Discover Agilent Bravo liquid
handling platform |
| Benchtop Workstations for Nucleic Acid Extraction | Agilent BenchCel Workstations provide complete automation of a variety of genomic sample preparation tasks. This workstation was developed as an automated solution for the isolation and purification of DNA in a Dellaporta-based nucleic acid extraction assay. | > Download Applications Highlight > Discover Agilent Bravo liquid
handling platform > Discover Agilent BenchCel
Microplate Handler > Discover Agilent Microplate
Centrifuge |
| Invitrogen ChargeSwitch EasyPlex gDNA kit using the gDNA isolation workstation | A flexible workstation providing high-throughput gDNA isolation • Up to 10 microplates can be processed in one run without user intervention • Microplate processing time is approximately 10 min per microplate (excluding incubation times) | >Download Application Bulletin > Agilent Bravo liquid handling
platform > BenchCel Microplate Handler > Discover Agilent PlateLoc Thermal
Microplate Sealer |